Department of Physics, Chemistry and Biology (IFM)

The moss Physcomitrella patens was used in this study to examine the expression patterns of GPI-anchored non-specific lipid transfer proteins (nsLTPs type G) during various abiotic stresses. This gene family consists of ten genes, PpLTPg1-10.

To mimic the stressful conditions during the water-to-land transition the stresses were conducted with either abscisic acid (ABA), salt (NaCl), mannitol (osmotic stress),copper (toxic ions), UV-B radiation (315-280 nm), cold and dehydration. Total RNA was extracted after the stresses and used to synthesize cDNA, which served as temple in the quantitiative real-time PCR (qRT-PCR) to analyze the gene expression. The genes expression levels were normalized to the housekeeping gene PpTUB1, encoding beta-tubulin 1. Additionally, a phylogenetic tree was constructed to establish the relationship within the gene family