Department of Physics, Chemistry and Biology (IFM)

Tissue was taken from rotavirus infected mice and uninfected mice. Samples were taken from three different parts of the small intestine (duodenum, jejunum and ileum) and at two different time points after infection (24h and 48h). Tissue was taken from twelve adult mice and twelve infant mice to see if there could be a difference in inflammation depending on the age. Cholit tissue was used as positive control. The tissue was sectioned and put on glass.

 

Immunohistochemistry was used for detection of inflammatory cells and inflammatory signals in the small intestine. Immunohistochemistry is the process where antigens are localized in cells or   tissue by using antibodies that bind specifically to the antigen. The antibody-antigen complex is then visualized by staining where the complex is conjugated to an enzyme. Antibodies were used for detection of TNF-alpha, rotavirus and CD45. CD45 is found on activated human B and T cells.