The course will give fundamental knowledge and experimental skills in the following areas of molecular Biology: Site-specific mutagenesis, gene cloning and PCR. After completing the course the student will have proficiency to:
- Understand basic laboratory techniques in molecular biology to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments.
- Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system
- Theoretical knowledge of DNA sequencing and DNA sequencing techniques as well as practical knowledge of techniques for purification of plasmid DNA
- Describe the function and applications of different enzymes involved in gene cloning experiments.
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Last updated: 08/08/13