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Genotyping of Dm-dNK transgenic mice
PCR
Dm-dNK expressing mice were created by crossing a Dm-dNK mouse (Dm-dNK+/-) with a wild-type mouse and the pups (approx. 14 days) were screened for the presence of the Dm-dNK gene by PCR (Figure 3). Clear bands were observed for the samples of mice 1, 2, 3 and 5, which confirmed the presence of the Dm-dNK gene.
Genotyping of Dm-dNK mice. Lane 1 – Fast Ruler DNA Ladder Middle Range (Fermentas) (M); Lanes 2-8 – DNA samples from #46-1 to #46-7; Lane 9 – Positive Control (P). The samples 1, 2, 3, & 5 (lanes 2, 3, 4 and 6 respectively) show clear band at approximately 850 bp. No band observed in samples 4, 6 and 7 (lanes 5, 7 and 8 respectively).
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Last updated:
05/22/11