The results from this study indicate that isoflurane has a strong effect on the capacity to form memory in mice. The unexpected outcome is that it does so in animals being anaesthetized with lower concentrations, while the ones undergoing deeper anaesthesia are not affected.

This work is important when considering the general assumption that volatile anaesthetics, such as isoflurane, are quickly eliminated by the body and have no secondary effects on the cognitive capacity of both humans and animals.

Further studies could implement the use of longer lasting procedures. In this work, animals were anaesthetized for one hour. Variability could be introduced on time as an extra factor to correlate with the different concentrations used.