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Publication list for Uno Carlsson

R. Mishra, L. Olofsson, M. Karlsson, U. Carlsson, I.A. Nicholls, P. Hammarström: A conformationally isoformic thermophilic protein with high kinetic unfolding barriers. Cell Mol. Life Sci. 65 (2008) 827-839 .

K. Almstedt, L.-G. Mårtensson, U. Carlsson and P. Hammarström: Thermodynamic interrogation of a folding disease - mutant mapping of position 107 in human carbonic anydrase II linked to marble brain disease Biochemistry 47 (2008) 1288–1298.

M. Karlsson and U. Carlsson: Adsorption at the liquid-solid Interface: Influence of protein stability on conformational changes. Encyclopedia of Surface and Colloidal Science 2nd edition   1 (2007) 1-16.

K. Carlsson, E. Persson, U. Carlsson and M. Svensson: Inhibitors of factor VIIa   the interface between the protease domain and tissue factor. Biochem. Biophys. Res. Commun. 349 (2006) 1111-1116.

F. Nayeri,   J. Xu, A. Abdiu, T. Nayeri, D. Aili , B. Liedberg and U. Carlsson:   Autocrine production of biologically active hepatocyte growth factor (HGF) by injured human skin. J. Dermatol. Sci. 43 (2006) 49-56.

M. Österlund, E. Persson,   U. Carlsson and P.-O. Freskgård, and Magdalena Svensson: Sequential coagulation factor VIIa domain binding to tissue factor. Biochem. Biophys. Res. Commun. 327 (2005) 1276-1282.

J. Wiréhn, K. Carlsson, A. Herland, E. Persson, U. Carlsson, M. Svensson and P. Hammarström: Activity , Folding, Misfolding and Aggregation in vitro of the Naturally Occurring Human Tissue Factor Mutant R200W. Biochemistry 44   (2005) 6755-67663.

  Karlsson, J. Ekeroth, H. Elwing and U. Carlsson: Reduction of irreversible protein Adsorption by stabilization: SPR studies of engineered stability variants of human carbonic anhydrase II to surfaces with fundamentally different properties. J. Biol. Chem. 280 (2005) 25558-25564.

M. Karlsson and U. Carlsson: Protein adsorption orientation in the light of fluorescent probes: Mapping of the interaction between site-directly labeled human carbonic anhydrase II and silica nanoparticles. Biophys. J. 88 (2005) 3536-3544.

M.Karlsson, L.-G. Mårtensson, C. Karlsson, C. and U. Carlsson: Denaturant-Assisted Formation of a Stabilizing Disulfide Bridge from Engineered Cysteines in Nonideal Conformations . Biochemistry 44 ( 2005 ) 3487-3493.  

M. Österlund, E, Persson, M. Svensson, U. Carlsson and P.-O. Freskgård: Transition state analysis of the complex between coagulation factor VIIa and tissue factor: Suggesting a sequential domain binding pathway. Biochem. Biophys. Res. Commun. 327 (2005) 789-793.

J. F. Knudsen, U. Carlsson, P. Hammarström, G. H. Sokol and   L. R. Cantilena: Cyclooxygenase - 2 inhibitors that inhibit the carbonic anhydrase II isozyme. Inflammation 28 (2005) 285-300.

K. Almstedt, M. Lundqvist, J. Carlsson, M. Karlsson, B. Persson, B.-H. Jonsson, U. Carlsson, P. Hammarström: Unfolding a Folding Disease: Folding, Misfolding and Aggregation of the Marble Brain Syndrome-associated Mutant H107Y of Human Carbonic Anhydrase II. J. Mol. Biol. 342 (2004) 619-633.

K. Borén, H. Grankvist, P. Hammarström and U. Carlsson: Reshaping the folding energy landscape by chloride salt: Impact on molten globule formation and aggregation behavior of carbonic anhydrase. FEBS Lett. 566 (2004) 95-99.

M. Karlsson, L.-G. Mårtensson,P. Olofsson and U. Carlsson: Circumnavigating misfolding traps in the energy landscape through protein engineering: Suppression of molten globule and aggregation in carbonic anhydrase. Biochemistry 43(2004) 6803-6807.

K. Carlsson, P.-O. Freskgård, E. Persson, U. Carlsson, and M. Svensson: Probing the interface between factor Xa and tissue factor in the quaternary complex tissue factor:factor VIIa:factor Xa:tissue factor pathway inhibitor. Eur. J. Biochem. 270 (2003) 2576-2582.

K. Carlsson, M. Österlund, E. Persson, P.-O. Freskgård, U. Carlsson, and M. Svensson:   Site-directed fluorescence probing to dissect the calcium-dependent association between soluble tissue factor and factor VIIa domains. Biochim. Biophys. Acta 1648 (2003) 12-16.

L.-G. Mårtensson, M. Karlsson, and U. Carlsson: Dramatic stabilization of the native state of human carbonic anhydrase II by an engineered disulfide bond. Biochemistry 41 (2002) 15867-15875.

M.T. Alam, T. Yamada, U. Carlsson and A. Ikai: The importance of being knotted: effects of the C-terminal knot structure on enzymatic and mechanical properties of bovine carbonic anhydrase II. FEBS Lett. 519 (2002) 35-40.

M. Huber, M. Lindgren, P. Hammarström, L.-G. Mårtensson, U. Carlsson, G.R. Eaton and S.S. Eaton: Phase memory relaxation times of spin labels in human carbonic anhydrase II: Pulsed EPR to determine spin label location. Biophysical Chemistry 94 (2001) 245-256.

M. Persson, J. R. Harbridge, P. Hammarström, R. Mitri, Lars-Göran Mårtensson, Uno Carlsson, Gareth R. Eaton, and Sandra S. Eaton: Comparison of EPR methods to determine distances between spin labels on human carbonic anhydrase II Biophys.J. 80 (2001) 2886-2897.

R. Owenius, M. Österlund, M. Svensson, M. Lindgren, P-O Freskgård, E. Persson and U. Carlsson,: Spin and fluorescent probing of the binding interface between tissue factor and factor VIIa at multiple sites Biophys. J. 81 (2001)   2357-2369.

M.Österlund, R. Owenius, K. Carlsson, U. Carlsson, E. Persson, M. Lindgren,   P.-O. Freskgård, and M. Svensson: Probing inhibitor-induced conformational changes along the interface between tissue factor and factor VIIa. Biochemistry 40 (2001) 9324 -9328.

S. Svedhem, K. Enander, M. Karlsson, H. Sjöbom, B. Liedberg, S. Löfås, L.-G. Mårtensson, S.-E. Sjöstrand, S. Svensson, U. Carlsson, and I. Lundström: Subtle differences in dissociation rates of interactions between destabilized human carbonic anhydrase II mutants and immobilized benzenesulfonamide inhibitors probed by a surface plasmon resonance biosensor. Anal. Biochem. 296 (2001) 188-196.

D. Andersson, U. Carlsson, P.-O. Freskgård: Contribution of Tryptophan Residues to the CD Spectrum of the Extracellular Domain of Human Tissue Factor: Application to folding studies and prediction of secondary structure. Europ. J. Biochem. 268 (2001) 1118-1128

D. Andersson, P. Hammarström and U. Carlsson: Cofactor Induced Refolding:Refolding of Molten Globule Carbonic Anhydrase Induced by Zn (II) and Co (II). Biochemistry 40 (2001) 2653-2661

P. Hammarström, R. Owenius, L.-G. Mårtensson, U. Carlsson, and M. Lindgren: High resolution probing of local conformational changes in proteins by the use of multiple labeling: Unfolding and aggregation of human carbonic anhydrase II monitored by spin, fluorescent and chemical reactivity probes. Biophys. J. 80 (2001) 2867-2885.

P. Hammarström, M. Persson, and U. Carlsson: Protein compactness measured by FRET: Human carbonic anhydrase II is considerably expanded by the interaction of GroEL. J. Biol. Chem. 276 (2001) 21765-21775.

M. Karlsson, L.-G. Mårtensson, B.-H. Jonsson and U. Carlsson: Adsorption of human carbonic anhydrase II variants to silica nanoparticles occurs stepwise: Binding is followed by successive conformational changes to a molten globule-like state. Langmuir 16 (2000) 8470-8479.

P. Hammarström, M. Persson, R. Owenius, M. Lindgren and U. Carlsson:   Protein substrate binding induces conformational changes in the chaperonin GroEL. A suggested mechanism for unfoldase activity. J. Biol. Chem. 275 (2000) 22832-22838.

M. Österlund, R. Owenius, E. Persson, M. Lindgren, U. Carlsson, P.-O. Freskgård, and M. Svensson: Spectroscopic probing of the influence of calcium and the Gla domain on the interaction between the first EGF domain in factor VIIa and tissue factor. Europ. J. Biochem. 267 (2000) 6204-6211.

U. Carlsson and B.-H. Jonsson: Folding and stability of human carbonic anhydrase II. In The Carbonic Anhydrases. New Horizons. (Chegwidden, Carter, and Edwards eds.) Birkhäuser Verlag, Basel. 2000, pp.241-262.

P. Hammarström and U. Carlsson: BREAKTHROUGHS AND VIEWS: Is the unfolded state the Rosetta stone of the protein folding problem? Biochem. Bioiphys. Res. Commun. 276 (2000) 393-398.

J.W. Vince, U. Carlsson, and A.F. Reithmeier: Localization of the Cl-/HCO3- anion exchanger binding site to the amino-terrmianl region of carbonic anhydrase II. Biochemistry 39 (2000) 13344-13349.

M. Persson, M. Lindgren, P. Hammarström, M. Svensson, B.-H. Jonsson and U. Carlsson: EPR Mapping of Interactions Between Spin-labeled Variants of Human Carbonic Anhydrase II and GroEL. Evidence for increased flexibility of the hydrophobic core by the interaction. Biochemistry 38 (1999) 432-441.

K. Borén and U. Carlsson: Characterization of a molten globule state of bovine carbonic anhydrase III: Loss of asymmetrical environment of the aromatic residues has a profound effect on both the near- and far-UV CD spectrum. Biochim. Biophys. Acta 1430 (1999) 111-118.

M. Österlund, R. Owenius, M. Lindgren, E. Persson, M. Svensson, P.-O. Freskgård and U. Carlsson: Properties of spin- and fluorescent labels at a receptor-ligand interface. Biohys. J. 77 (1999) 2237-2250.

P. Hammarström, M. Persson, P.-O. Freskgård, L.-G. Mårtensson, D. Andersson, B.-H. Jonsson and U. Carlsson: Structural maping of an aggregation nucleation site in a molten-globule intermediate. J. Biol. Chem. 274(1999) 32897-32903.

P. Billsten, U. Carlsson, B.-H. Jonsson, G. Olofsson, F. Höök and H. Elwing: Conformation of human carbonic anhydrase II variants adsorbed to silica nanoparticles. Lamgmuir 15 (1999) 6395-6399.

P. Billsten, U. Carlsson and H. Elwing: Studies on the conformation of adsorbed proteins               with the use of nanoparticle technology in Biopolymers at Interfaces (M.Malmsten ed.) Marcel Dekker Inc., New York   1998, pp. 627-650.

D. Andersson, P.-O. Freskgård, B.-H. Jonsson and U. Carlsson: Formation of local asymmetric structures in the slow folding reaction of human carbonic anhydrase II. Biochemistry 36 (1997) 4623-4630

P. Billsten, P.-O. Freskgård, U. Carlsson, B.-H. Jonsson and H. Elwing: Adsorption to silica nanoparticles of human carbonic anhydrase II and truncated forms induce molten globule structure. FEBS Lett. 402 (1997) 67-72

P. Jonasson, G. Aronsson, U. Carlsson and B.-H. Jonsson: Tertiary structure formation at tryptophan side-chains in the refolding of human carbonic anhydrase II. Biochemistry 361 (1997) 5142-5148.

K. Borén, P.-O. Freskgård and U. Carlsson: A comparative CD study of carbonic anhydrase isoenzymes with different number of tryptophans. Impact on calculation of secondary structure content. Protein Sci. 5 (1996) 2479-2484.

M. Persson, U. Carlsson and N. Bergenhem: GroEL reversibly binds to, and causes rapid inactivation of, human carbonic anhydrase II at high temperatures. Biochim. Biophys. Acta 1298 (1996) 191-198.

M. Persson, U. Carlsson and N. Bergenhem: GroEL provides a folding pathway with lower apparent activation energy compared to spontaneous refolding of human carbonic anhydrase II. FEBS Lett. 411 (1997) 43-47.

P. Hammarström, B. Kalman, B.-H. Jonsson and U. Carlsson: Investigation of a stable folding intermediate by the use of pyrene eximers. FEBS Lett. 420 (1997) 63-68.

M.Lindgren, G.R. Eaton, S.S. Eaton, B.-H. Jonsson, P. Hammarström, M. Svensson and U. Carlsson: Electron spin echo as a probe of nitroxide environment in spin labeled mutants of human carbonic anhydrase II. J. Chem. Soc. Perkin. Trans. 2 (1997) 2549-2554.

M. Lindgren, M. Svensson, P.-O. Freskgård and U. Carlsson:Characterization of a folding intermediate of human carbonic anhydrase II. Probing local mobility by EPR. Biophys. J. 69 (1995) 202-213.

N.C.H. Bergenhem, B. D. Schlyera, D.G. Steela, A. Gafni, U. Carlsson and B.-H. Jonsson: Lack of correspondence between the room-temperature phosphorescence decay component and TrpCys or TrpPhe mutants of human carbonic anhydrase II. FEBS Lett 1994 (353) 177-179.

M. Svensson, P. Jonasson, P.-O. Freskgard, B.-H. Jonsson, M. Lindgren, L.-G. Mårtensson, M. Gentile, K. Boren and U. Carlsson. Mapping the folding intermediate of human carbonic anhydrase II. Probing substructure by chemical reactivity and spin- and fluorescent labeling of engineered cysteine residues. Biochemistry 34 (1995) 8606-8620.

G. Aronsson, L.-G. Mårtensson, U. Carlsson and B.-H. Jonsson: Folding of the N-terminus of human carbonic anhydrase II. Biochemistry 34 (1995) 2153-2162.

U. Carlsson and B.-H. Jonsson: Folding of -sheet proteins. Current Opin. Struct. Biol. 5 (1995) 482-487.

M. Lindgren, M. Svensson, P.-O. Freskgård, U. Carlsson, B.-H. Jonsson, L.-G. Mårtensson              and P. Jonasson: Stability measurements on spin-labelled mutants of carbonic anhydrase II. EPR characterization of folding intermediates. Magnetic Resonance in Medicine 6 (1995) 187-189.

M. Persson, G. Aronsson, N. Bergenhem, P.-O. Freskgård, B.-H. Jonsson, B. P. Surin, M. D. Spangfort and U. Carlsson: GroEL/ES-mediated refolding of human carbonic anhydrase II: Role of N-terminal helices as recognition motifs for GroEL. Biochim. Biophys. Acta 1247 (1995) 195-200.

L.-G. Mårtensson, P. Jonasson, P.-O. Freskgård, M. Svensson, U. Carlsson, and B.-H. Jonsson : Contribution of individual tryptophan residues to the fluorescence spectrum of native and denatured forms of human carbonic anhydrase II: Use of acrylamide quenching for structural analysis. Biochemistry 34 (1995) 1011-1021.

P.-O. Freskgård, L.-G. Mårtensson, P. Jonasson, B.-H. Jonsson and U. Carlsson: Assignment of the contribution of the tryptophan residues to the circular dichroism spectrum of human carbonic anhydrase II. Biochemistry 33 (1994) 14281-14288.

L.-G. Mårtensson, B.-H. Jonsson, P.-O. Freskgård, A. Kihlgren, M. Svensson and U. Carlsson: Characterization of folding intermediates of human carbonic anhydrase II. Probing substructure by chemical labeling of SH-groups introduced by site-directed mutagenesis. Biochemistry 32 (1993) 224-231.

  M. Lindgren, M. Svensson, P.-O. Freskgård, U. Carlsson, B.-H. Jonsson, L.-G. Mårtensson and P. Jonasson: Probing local mobility in carbonic anhydrase. EPR of spin-labeled SH groups introduced by site-directed mutagenesis. J. Chem. Soc. Perkin. Trans.2 (1993) 2003-2007.

  U. Carlsson, P.-O. Freskgård, B.-H. Jonsson, A. Kihlgren, L.-G. Mårtensson and M. Svensson: Study of folding intermediates by chemical labeling of cysteine mutants. Protein Engineering 6 Suppl. (1993) 9.

 C. Fransson, P.-O. Freskgård, H. Herbertsson, Å. Johansson, P. Jonasson, L-G. Mårtensson, M. Svensson, B.-H. Jonsson and U. Carlsson: Cis-trans isomerization is rate-determining in the reactivation of denatured human bonic anhydrase II as evidenced by proline isomerase FEBS Lett. 296 (1992) 90-94.

  U. Carlsson, P.-O. Freskgård and S.C.T. Svensson: A simple and efficient synthesis of the inducer IPTG made for inexpensive heterologous protein production using the lac-promoter. Protein Engineering 4 (1992) 1019-1020.

  P.-O. Freskgård, N. Bergenhem and U. Carlsson: A versatile stopped-flow apparatus with adjustable pistons. Analytica Chimica Acta 269 (1992) 143-148.

  P.-O. Freskgård, N. Bergenhem, B.-H. Jonsson, M. Svensson and U. Carlsson: Isomerase and chaperon activity of prolyl isomerase in the folding of carbonic anhydrase. Science 258 (1992) 466-468.

M. Andersson, P.-O. Freskgård, A. Kihlgren, N. Bergenhem and U. Carlsson. L.-G. Mårtensson and B.-H. Jonsson: The effect of substituting histidine 64 in human carbonic anhydrase II on stability of the enzyme. In "Carbonic Anhydrase. From Biochemistry and Genetics to Physiology and Clinical Medicine." (F. Botré, G. Gros, and B.T. Storey, eds.) Verlag Chemie, Weinheim, 1991 pp. 119-125.

 L.-G. Mårtensson, B.-H. Jonsson, M. Andersson, A. Kihlgren, N. Bergenhem and U. Carlsson: Role of an evolutionarily invariant serine for the stability of human carbonic anhydrase II. Biochim. Biophys. Acta 1118 (1991) 179-186.

  P. O. Freskgård, U. Carlsson, L.-G. Mårtensson, and B.-H. Jonsson: Folding around the the C-terminus of human carbonic anhydrase II. Kinetic characterization by use of a chemically reactive SH-group introduced by protein engineering. FEBS Lett. 289 (1991) 117-122.

  K. Borén, M. Larsson, N. Bergenhem, and U. Carlsson: Rapid ion-exchange chromatography for preparative separtaion of proteins. Application on bovine carbonic anhydrase III from skeletal muscle. J. Chromatogr. 588 (1991) 139-145.

  G. Asplund, H. Borén, U. Carlsson, and A. Grimvall: Soil peroxidase-mediated chlorination of fulvic acid. In Humic substances in the aquatic and terrestrial environment. (B. Allard, H. Borén, and A. Grimvall eds.) Springer Verlag, 1991 pp. 475-483.

N. Bergenhem, U. Carlsson and J-Å Karlsson: Evidence for an initial fast nucleation process in the folding of human carbonic anhydrase I. Int. J. Peptide and Protein Res. 33 (1989) 140-145.

  N. Bergenhem and U. Carlsson: Some new by-products in primary structure analysis with dimethylaminoazobenzene isothiocyanate as an aid in the thin-layer chromatographic identification of residues. J. Chromatogr. 472 (1989) 318-320.

N. Bergenhem and U. Carlsson: Partial amino acid sequence of erythrocyte carbonic anhydrase from tiger shark. Comp. Biochem. Physiol. 95 (1989) 205-213.

  N. Bergenhem and U. Carlsson:   The role of the metal ion in the refolding of denatured bovine Co(II)-carbonic anhydrase II. Biochim. Biophys. Acta 998 (1989) 277-285.

  N. Bergenhem, U. Carlsson, L-E Ellow and S. Rosén: Rapid Ion Exchange Chromatography for Preparative Separations of Proteins. III. Application to purification of bovine factor X and prothrombin. J. Chromatogr. 403 (1987) 383-387.

N. Bergenhem, U. Carlsson and L. Strid: The existence of glutathione and cysteine disulfide-linked to erythrocyte   carbonic anhydrase from tiger shark. Biochem. Biophys. Acta 871 (1986) 55-60.

  N. Bergenhem and U. Carlsson: Rapid Ion Exchange Chromatography for Preparative Separations of Proteins.II. Application to anaerobic purification of ribulose-1,5-bisphosphate carboxylase/oxygenase. J. Chromatogr. 360 (1986) 279-281.

N. Bergenhem, U. Carlsson and K.Klasson: Rapid Ion Exchange Chromatography for Preparative Separations of Proteins. Application to porcine and bovine carbonic anhydrases. J. Chromatogr. 319 (1985) 59-65.

  N. Bergenhem and U. Carlsson: Denaturation and renaturation of bovine and human cobalt-carbonic anhydrases in guanidineHCl, Annals New York Academy of Sciences 429 (1984) 137-139.

  N. Bergenhem, U. Carlsson, G. Lind and I.-M. Åstrand: Denaturation and reactivation of bovine and human cobalt carbonic anhydrases in guanidine hydrochloride, Acta Chem. Scand. B 37 (1983) 244-246.

N. Bergenhem, U. Carlsson and C. Hansson: Preparative affinity Electrophoresis: Application to human erythrocyte carbonic anhydrase, Anal. Biochem. 134 (1983) 259-263.

  U. Carlsson, B. Kjellström and B. Antonsson: Purification and properties of cyclostome carbonic anhydrase from erythrocytes of hagfish, Biochim. Biophys. Acta, 612 (1980) 160-170.

  L. Berglund, U. Carlsson and B. Kjellström: Cyclostome carbonic anhydrase: Purification and some properties of the enzyme from erythrocytes of lamprey, Acta Chem. Scand. B 34 (1980) 227-228.

J.L. Sudmeier, J.L. Evelhoch and U. Carlsson: Effect of bicarbonate and inhibitors on the 113cadmium NMR signals in zinc-replaced human carbonic anhydrase in Magnetic resonance and related phenomena (E. Kundla, E. Lippmaa and T. Saluvere, eds.), Springer-Verlag, Berlin, 1979, p.541.

U Carlsson, R. Aasa, L.E. Henderson, B.-H. Jonsson and S. Lindskog: Paramagnetic and fluorescent probes attached to "buried" sulfhydryl groups in human carbonic anhydrases. Application to inhibitor binding, denaturation and refolding. Eur. J. Biochem. 52 (1975) 25-36.

U. Carlsson: Studies on carbonic anhydrase isoenzymes. With special reference to dynamic aspects of protein structure. Doctoral Thesis, Göteborg, 1974.

U. Carlsson, L.E. Henderson, P.O. Nyman and T. Samuelsson: Studies on the influence of carboxyl-terminal amino acid residues on the activity and stability of human erythrocyte carbonic anhydrase B, FEBS Lett. 48 (1974) 167-171.

U. Carlsson, L-E. Henderson and S. Lindskog: Denaturation and reactivation of human carbonic anhydrase in guanidine hydrochloride and urea, Biochim. Biophys. Acta, 310 (1973) 376-387.

U. Carlsson, U. Hannestad and S. Lindskog: Purification and some properties of erythrocyte carbonicanhydrase from the European moose, Biochim. Biophys. Acta 327 (1973) 515-527.

U. Carlsson, S. Lindskog, E. Andersson, O. Lindqvist and G. Olsson: Crystallization and preliminary X-ray investigation of bovine erythrocyte carbonic anhydrase, J. Mol. Biol. 80 (1973) 373-375.


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