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Biosensors and Bioelectronics Laboratory

Lab L202

In our dedicated Biosensors and Bioelectronics Laboratory lab we develop, fabricate and evaluate our biosensor and bioelectronic devices. We have a full range of scanning voltammetric and impedance technologies, and surface characterisation tools. These includes several potentiostats (Ivium CompactStats, IviumStat, and EMstats) with accessories such as multiplexers and rotating disc electrodes. In addition, we have dedicated equipment for actuator research such as a special designed laser scanner (Mitutoyo LSM501H) set-up for non-contact thickness measurements, and a digital videocamera.

Laboratory responsible: Edwin Jager

Zeta Potential and Particle Size Analyzer

Zetasizer Nano ZS90 is an integrated system for the measurement of particle size and molecular size at a 90 degree scattering angle using Dynamic Light Scattering, also with the ability to measure zeta potential and electrophoretic mobility using Laser Doppler Microelectrophoresis, and molecular weight using Static Light Scattering. The Zetasizer Nano ZS90 incorporates three techniques in a single compact unit, and has a range of options and accessories to optimize and simplify the measurement of different sample types.

 Dynamic Light Scattering at a scattering angle of 90 degrees is used to measure particle size and molecule size. This technique measures the diffusion of particles moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship.

Laser Doppler Micro-electrophoresis is the technique used to measure zeta potential. An electric field is applied to a solution of molecules or a dispersion of particles, which will then move with a velocity related to their zeta potential. This velocity is measured using a patented laser interferometric technique called M3-PALS (Phase analysis Light Scattering). This enables the calculation of electrophoretic mobility, and from this, the zeta potential for the accurate measurement of a wide range of sample types and dispersion media including high salt concentrations and non-aqueous dispersants.

Static Light Scattering is used to determine the molecular weight of proteins and polymers. In this technique, the scattering intensity of a number of concentrations of the sample is measured, and used to construct a Debye plot. From this the average molecular weight and second virial coefficient can be calculated. This measurement is very demanding on the stability of the whole system, and means that every element of the design has been optimized to ensure accuracy and repeatability.

Facilities for Lateral Flow Strip Tests

The Biosensor & Bioelectronics Centre houses the core equipment (Iso-Flow Dispenser, Membrane Cutter and Assembly Roller) to accommodate for small to medium scale prototyping and production of lateral flow strip tests.

The Iso-Flow Dispenser dispenses reagents and colloidal gold onto membrane. The machine comes standard with three pumps, two needles (control and test lines) and one atomizer nozzle for colloidal gold dispensing. The operator places pre-laminated membranes onto the machine bed and enters the appropriate variables into the computer control interface for dispensing.

The Member Cutter is used for cutting assembled membranes into individual tests. The operator loads the card and engages the machine. The machine offers a convenient touch pad to change cutting widths quickly and easily.   Once loaded the cutter is self-feeding and features a safety cutoff that disengages the cutter if the blade guard is lifted during operation.

The Assembly Roller is a simple machine that seals cassettes. After a test strip is placed inside the plastic cassette housing and the housing is loosely closed the operator slides the cassette through the rotating press on the roller. The roller then seals the cassette tightly.

LSM 501H laser scanner

The LSM501H and controller LSM6100 is a non-contact measuring unit. Using a high speed scanning laser beam,the Laser Scan Micrometer can accurately measure workpieces which are difficult for conventional instruments, such as brittle or elastic parts, hot parts, parts which need to be kept clean, and soft parts which may suffer dimensional changes due to measuring force. Transparent part measurement is also possible. Even measurement of objects submerged under water is possibl. We employ the LSM to do non-contact, in situ measurements of the volume change of PPy. PPy films are actuated in an aqueous electrolyte by means of a potentiostat. The LSM detects the size of an object placed within its beam by measuring the “cast shadow”, equal to the PPy diameter, on the LSM detector with a sensitivity of 0.01 µm and reproducibility of ±0.05 µm. As the PPy film expands or contracts, the shadow cast on the detector changes accordingly and the thickness change is recorded. such as electrochemically induced volume change of polypyrrole.

Device Physics Lab (IFM Clean Room)

In addition we fabricate devices in the shared IFM clean room that is a class 1000 clean room with full microfabrication capabilities, including photolithography line, mask fabrication equipment, wet and dry etching (RIE), vapour deposition (such as thermal evaporation and CVD). A separate area of the clean room is dedicated to soft lithography.

Printed Electronics Arena

The Biosensors and Bioelectronics Centre makes extensive use of the Printed Electronics Arena, which is a national facility located at our Norrköping Campus

Facilities at IFM and Linköping University

We have access to the facilities at IFM and Linköping University that are open for all users. All the equipment required to carry out our research is conveniently located and is of high or state-of-the art standard. Facilities include: Electrochemistry Laboratories: full range of scanning voltammetric and impedance technologies, surface characterisation tools, microelectrode and array fabrication including silcon microfabrication and printing technologies including screen printing, ink-jet and reel to reel. Soft Lithography Laboratories: soft lithography for organic electronics and biotechnology, and also allows characterisation of microstructures utilising fluids (microfluidics). Biochip lab with metallisation processes equipment as well as electrochemical work stations. Molecular and surface spectroscopy labs (FT-IR, Raman, XPS), Imaging/Nano laboratories: (SPR, Imaging SPR, Spectroscopic/imaging ellipsometry, DLS) -Electron Spin Resonance/Relaxivity measurements AFM. Strategic Importance Sensor laboratories: (Biacore, QCM-D, electrochemistry, impedance) - Organic electronics device processing (glove box system with evaporator & device charact.) - Optical spectroscopy (transmission, emission) - Probe station for vacuum studies of organic electronic devices under temperature control. Large Area Electronics laboratories: Hosts activities for organic (plastic) electronics aimed for exploring plastic electronics added to unconventional carriers, such plastic foils and paper. - Expanded recently to include an array of printing, coating and lamination techniques for the development of reel-to-reel manufacturing of electronics (Printed Electronics Arena). - Combines lithography and vacuum processing tools with those of printing manufacturing with processing lines for 4-inch substrates, 24×24” panels, and rolls (flexible substrates). Electron microscopy Laboratories: Delocalisation-free (Cs-corrected) TEM (under procurement), FEG-TEM/EELS/EDX, HREM, in situ Nanoindentation, FIB/FEG-SEM, FEG-SEM/Helium- Cryo-Cathodoluminescence/EDX/EBSB, FEG-SEM/WDX. XRD-Laboratories.: World leading dedicated laboratory for thin film work, texture, stress, and epitaxy, Powder, Reciprocal space map, In situ Furnace, Texture, Stress. Access to state-of-the-art materials science and surface diffractometer at Beamline I811 at MAX-II, a Swedish 3rd generation synchrotron source in Lund. Spectroscopic ellipsometry: World-leading laboratory for optical analysis of surfaces, films and nanostructures in the spectral range 190 nm to 33 μm. Generalised, infrared, time-resolved & full Mueller-matrix (only a few systems in the world) ellipsometers. Facilities for solid/liquid in situ, microspot (50 μm), time-resolved, internal reflection and RT-300 °C studies - Scanning Probe Microscopy: Nanoscope III system with tapping mode capability Digital Instruments Dimension 3100; Digital Instruments Enviroscope 2 Nanonics SNOM & NSOM systems (one with low temp 4 K capability) STM /MBE/sputtering system for in situ studies of nanostructures on surfaces. Auger Electron Spectroscopy and XPS Laboratories.: Fison high lateral res. scanning Auger electron spectrometer (XPS & REELS). AFM – Application for a Strategic Research Area Grant in Materials Science from Linköping University 26 Part B. Strategic Importance Scienta ESCA 200, for high resolution XPS (0.25 eV) and UPS. High-quality UPS. Spin Resonance: CW and pulsed electron spin resonance (ESR) and ODMR at 9, 35, 95 GHz. Materials Modelling Laboratory: New computer systems under procurement (Abrikosov, Stafström et al.) Linux cluster for physical computations (“Green”) with workstations for visualizations. Simulation Tools and Software: Commercial and open source state-of-the-art software for ab initio simulations (VASP, FP-LMTO, Quantum Espresso, EXCITING, etc.) Dalton 1.2.1 for calculating molecular properties at HF,DFT,MCSCF,CC theory level. Gaussian 03 Tools for classical molecular dynamics and statistical mechanics simulation in-house development. Inorganic and Organic Chemistry laboratories: facilitating the synthesis and characterisation of materials and including a range of NMR machines up to 800 MHz, IR, MALDI-Tof, CD and differential calorimetry.

Responsible for this page: Martin Wing Cheung Mak
Last updated: 06/16/15