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Protein design and studies of protein interactions


IgG-Fc interactions of relevance to rheumatoid arthritis

These projects are run in close collaboration with the team of professor Thomas Skogh at the rheumatology unit, IKE, LiU.

Surface design for studies of cell adhesion and activation

This project aims at creating molecular models for detailed understanding of the interaction between IgG complexes and the Fcγ receptor, present in the cell membrane of all immunologically active cells and mediating a variety of immune responses. Monoclonal hIgG is immobilized at different densities in an oriented fashion on a self-assembled monolayer of oligo(ethylene glycol)-containing alkane thiolates on gold. This model is then used to study the adhesion propensity and state of activation of human monocytes and macrophages.

Related publications:

Kanmert D, Enocsson H, Wetterö J, Kastbom A, Skogh T, and Enander K: "Designed surface with tunable IgG density as an in vitro model for immune complex mediated stimulation of leukocytes." Langmuir 26: 3493–3497 (2010) Abstract

Development of immunoassay for IgG-RF

An immunoassay is being designed for the IgG variant of rheumatoid factor (RF), autoantibodies specific for the Fc part of IgG molecules and notoriously difficult to detect by traditional sandwich ELISA systems due to a high degree of false positives. We are addressing this problem by a molecular design approach which includes modification and expression of IgG-Fc in the yeast host Pichia pastoris. A panel of IgG-Fc of different subclasses will be used to profile the specificity of IgG-RF in patients suffering from rheumatoid arthritis.

Contact: Daniel Kanmert (dakan@ifm.liu.se)

Responsible for this page: Erik Martinsson
Last updated: 06/01/10